Biotecnología

Biotecnología

Expression of a Copper Activated Xylanase in Yeast: Location of the His-Tag in the Protein Significantly Affects the Enzymatic Properties
30/11/2022 - Abstract A copper activated xylanase produced by E. coli BL21 was expressed in Pichia pastoris using the pGAPZ?B expression vector. Two recombinant GH11 xylanase forms were...
Immunogenicity of inactivated coronavirus disease 2019 vaccines in patients with chronic hepatitis B undergoing antiviral therapy
30/11/2022 - ObjectivesTo investigate the effect and its mechanisms of different antiviral agents on the immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines in patients with...
Short-Term Interaction with Endothelial Cells Enhances Angiogenic Activity of Growth-Arrested Mesenchymal Stromal Cells In Vitro and In Ovo
30/11/2022 - We compared angiogenic effects of conditioned medium from mesenchymal stromal cell (MSC) monoculture and co-culture of MSC with endothelial cells (EC). Conditioned medium from 24-h EC-MSC...
Recent Advances in Antibiotic-Free Markers; Novel Technologies to Enhance Safe Human Food Production in the World
30/11/2022 - Abstract Crops that have been genetically modified (GM) include additional beneficial traits that present fresh approaches to boosting yield productivity and sustainable...
Fecal microbiota transplantation restores normal fecal composition and delays malignant development of mild chronic kidney disease in rats
30/11/2022 - Chronic kidney disease (CKD) is associated with gut microbiome dysbiosis, but the role of intestinal flora in CKD treatment remains to be elucidated. Fecal microbiota transplantation (FMT) can be...
Production of Stem Cell Antigen 1 Sca-1/Ly-6A/E by Freshly Isolated NK Cells Cultured with Relevant Cytokines
30/11/2022 - Activated NK cells in appropriate conditions are known to express stem cell antigen 1 (Sca-1/Ly-6A/E). To investigate its production, NK cells isolated from mouse spleens were incubated ex vivo in...
Enhanced strength-ductility-toughness synergy in an HSLA steel with multi-gradient ultrafine grained structure by adopting a two-stage rolling coupling inter-pass ultra-fast cooling process
30/11/2022 - In this work, a multi-gradient ultrafine grain (GUFG) structured high strength low alloy (HSLA) steel was successfully prepared by adopting a two-stage rolling coupling ultra-fast cooling process....
Scrophulariae Radix-Atractylodes sinensis pair and metformin inhibit inflammation by modulating gut microbiota of high-fat diet/streptozotocin-induced diabetes in rats
30/11/2022 - IntroductionType 2 mellitus (T2DM), a chronic metabolic disorder, causes severe impairment of patients’ quality of life and has attracted global attention. Many studies have suggested the...
Silatrane-Sulfonamide Hybrids as Promising Antibacterial Agents
30/11/2022 - At the A. E. Favorsky Irkutsk Institute of Chemistry, a series of silatrane-sulfonamide hybrids 1a-d and 2a-d was synthesized. The antibacterial activity of...
A Chinese herbs complex ameliorates gut microbiota dysbiosis induced by intermittent cold exposure in female rats
30/11/2022 - Cold is a common source of stress in the alpine areas of northern China. It affects the microbial community, resulting in the invasion of pathogenic microorganisms and intestinal diseases. In...
Cephalosporins as key lead generation beta-lactam antibiotics
30/11/2022 - Abstract Antibiotics are antibacterial compounds that interfere with bacterial growth, without harming the infected eukaryotic host. Among the clinical agents, beta-lactams...
A case report of leishmaniosis with primary oral manifestation in a cat
30/11/2022 - A case of leishmaniosis with primary oral manifestations was reported in a 10-year-old neutered domestic shorthair cat. The primary lesion was a maxillary nodular lesion, painful with spontaneous...
Salmonella enterica serovar Cerro displays a phylogenetic structure and genomic features consistent with virulence attenuation and adaptation to cattle
30/11/2022 - Salmonella enterica subsp. enterica (S.) serovar Cerro is rarely isolated from human clinical cases of salmonellosis but represents the most common serovar isolated from cattle without clinical...
RAP44 phage integrase-guided 50K genomic island integration in Riemerella anatipestifer
30/11/2022 - Bacteriophages are viruses that infect bacteria. Bacteria and bacteriophages have been fighting for survival. Over time, the evolution of both populations has been affected. Pathogenic...
Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigs
30/11/2022 - IntroductionThe establishment of intestinal microbiota and the maintenance of its equilibrium structure plays an important role in Tibetan pigs during different growth stages. Understanding the...
Knowledge, attitudes, and practices on camel respiratory diseases and conditions in Garissa and Isiolo, Kenya
30/11/2022 - BackgroundLivestock farmers' attitudes, practices, and behaviors are major factors in infection prevention and control of animal diseases. Kenya has the fourth largest global camel population, and...
TMPRSS2 gene polymorphism common in East Asians confers decreased COVID-19 susceptibility
30/11/2022 - COVID-19 has a wide range of clinical presentations, and the susceptibility to SARS-CoV-2 infection and the mortality rate also vary by region and ethnicity. Here, we found that rs12329760 in the...
Serological survey of SARS-CoV-2 in companion animals in China
30/11/2022 - Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) can be transmitted from human to companion animals. The national wide serological surveillance against SARS-CoV-2 was conducted among...
Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering
30/11/2022 - ta name="citation_online_date" content="2Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering - ScienceDirect
Surgical treatment of cranial cruciate ligament disease in dogs using Tibial Plateau Leveling Osteotomy or Tibial Tuberosity Advancement–A systematic review with a meta-analytic approach
30/11/2022 - Tibial Plateau Leveling Osteotomy (TPLO) or Tibial Tuberosity Advancement (TTA) are commonly used surgical techniques for correction of cranial cruciate ligament (CCL) rupture in dogs. This...