Biotecnología

Biotecnología

A Review of Methodologies for the Detection, Quantitation, and Localization of Free Cysteine in Recombinant Proteins: A Focus on Therapeutic Monoclonal Antibodies
27/06/2022 - Free-cysteine residues in recombinant biotherapeutics such as monoclonal antibodies can arise from incorrect cellular processing of disulfide bonds during synthesis or by reduction of disulfide...
Improvement of Sleeping Beauty Transposon System Enabling Efficient and Stable Protein Production
27/06/2022 - Abstract The most essential goal in the field of biopharmaceuticals is to develop cell lines with higher protein yields. To this goal, the Sleeping Beauty (SB) transposon-based...
Impacts of Delivery Mode and Maternal Factors on Neonatal Oral Microbiota
27/06/2022 - ObjectivesInitial oral microbial colonization has complicatedly interacted with growth and development. The aim of our study was to discover links between oral microbiota community structure and...
Antioxidant Mechanism of Lactiplantibacillus plantarum KM1 Under H2O2 Stress by Proteomics Analysis
27/06/2022 - Lactiplantibacillus plantarum KM1 was screened from natural fermented products, which had probiotic properties and antioxidant function. The survival rate of L. plantarum KM1 was 78.26% at 5 mM...
Human Claudin-7 cis-Interactions Are Not Crucial for Membrane-Membrane (Trans-) Interactions
27/06/2022 - Human Claudin-7 (Cldn7) is a member of the Claudin (Cldn) superfamily. In vivo, these proteins form tight junctions, which establish constricted connections between cells. Cldns oligomerize within...
Comprehensive Characterization of CK1?-Mediated Tau Phosphorylation in Alzheimer’s Disease
27/06/2022 - A main pathological event in Alzheimer’s disease is the generation of neurofibrillary tangles originating from hyperphosphorylated and subsequently aggregated tau proteins. Previous reports...
A Method for Assessing the Robustness of Protein Structures by Randomizing Packing Interactions
27/06/2022 - Many single-domain proteins are not only stable and water-soluble, but they also populate few to no intermediates during folding. This reduces interactions between partially folded proteins,...
Theabrownin in Black Tea Suppresses UVB-induced Matrix Metalloproteinase-1 Expression in HaCaT Keratinocytes
27/06/2022 - Abstract Theabrownin is a heterogeneous polyphenolic compound, obtained from fermented dark teas, with many bioactive functions. However, the role of theabrownin in skin...
Mastoparans: A Group of Multifunctional ?-Helical Peptides With Promising Therapeutic Properties
24/06/2022 - Biologically active peptides have been attracting increasing attention, whether to improve the understanding of their mechanisms of action or in the search for new therapeutic drugs. Wasp venoms...
Proteolysis at the Archaeal Membrane: Advances on the Biological Function and Natural Targets of Membrane-Localized Proteases in Haloferax volcanii
24/06/2022 - Proteolysis plays a fundamental role in many processes that occur within the cellular membrane including protein quality control, protein export, cell signaling, biogenesis of the cell envelope...
Antimicrobial peptides: Sustainable application informed by evolutionary constraints
24/06/2022 - https://www.sciencedirect.com/science/article/pii/S0734975022001082?dgcid=rss_sd_allhttps://www.sciencedirect.com/science/article/pii/S0734975022001082Bacillus licheniformis: The unexplored...
Rapid, Sensitive, and Species-Specific Detection of Conventional and Recombinant Herpesvirus of Turkeys Vaccines Using Loop-Mediated Isothermal Amplification Coupled With a Lateral Flow Device Readout
24/06/2022 - Marek's disease, an economically important disease of chickens caused by virulent serotype 1 strains of the Mardivirus Marek's disease virus (MDV-1), is effectively controlled in the field by live...
Effects of Galactomannan Oligosaccharides on Growth Performance, Mycotoxin Detoxification, Serum Biochemistry, and Hematology of Goats Fed Mycotoxins-Contaminated Diets
24/06/2022 - This study was conducted to investigate the protective effects of mycotoxin adsorbent galactomannan oligosaccharides (GMOS) on growth performance, fermentation parameters, mycotoxins residues,...
The First Genomic Analysis of Visna/Maedi Virus Isolates in China
24/06/2022 - Visna/Maedi virus (VMV) is a neglected pathogen that damages sheep and goats' nervous and respiratory systems. The virus was discovered 80 years ago and has been endemic in China for nearly four...
Approaches to Using the Chameleon: Robust, Automated, Fast-Plunge cryoEM Specimen Preparation
24/06/2022 - The specimen preparation process is a key determinant in the success of any cryo electron microscopy (cryoEM) structural study and until recently had remained largely unchanged from the initial...
Derivation of splice junction-specific antibodies using a unique hapten targeting strategy and directed evolution
24/06/2022 - Alternative splicing of RNA occurs frequently in eukaryotic cells and can result in multiple protein isoforms that are nearly identical in amino acid sequence, but have unique biological roles....
Cilia-Localized Counterregulatory Signals as Drivers of Renal Cystogenesis
24/06/2022 - Primary cilia play counterregulatory roles in cystogenesis—they inhibit cyst formation in the normal renal tubule but promote cyst growth when the function of polycystins is impaired. Key...
Exosomes as Theranostic Targets: Implications for the Clinical Prognosis of Aggressive Cancers
24/06/2022 - Exosomes are extracellular vesicles produced by various cell types and extensively distributed in physiological fluids. Because of their significant role in cancer progression, they have been a...
Author Correction: Influence of extracellular protein isolated from fish gut associated bacteria as an enhancer of growth and innate immune system in Mugil cephalus
24/06/2022 - Scientific Reports, Published online: 23 June 2022; doi:10.1038/s41598-022-15044-6Author Correction: Influence...
OOP-ESEEM Spectroscopy: Accuracies of Distances of Spin-Correlated Radical Pairs in Biomolecules
24/06/2022 - In addition to the commonly used electron–electron double resonance (ELDOR) technique, there are several other electron paramagnetic resonance (EPR) methods by which structure information can be...